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생물학적 환경정화를 위한 고농도 페놀에서 생육할 수 있는

세균의 분리 및 특성

홍길동*ㆍ이몽룡1)ㆍ성춘향2)

세계대학교 환경공학과, 1)세계대학교 생물공학과, 2)세계대학교 화학공학과

Isolation and Characterization of Bacteria Able to Grow with Phenol at High Concentrations for Bioremediation

영문 제목의 첫글자는 대문자,

이하 각 단어의 첫글자는 대문자 사용(전치사, 관사, 접속사 제외)

Gil-Dong Hong*, Mong-Yong Lee1), Chun-Hyang Seong2)

Department of Environmental Engineering, Sekye University, Busan 47514, Korea

1)Department of Biotechnology, Sekye University, Busan 47514, Korea

2)Department of Chemical Engineering, Sekye University, Busan 47514, Korea

Abstract

For the biological treatment of industrial wastewater containing high concentration of phenol, isolation and characterization of phenol-degrading bacterium were carried out. A bacterial strain P2 capable of degrading phenol was isolated from contaminated soils by enrichment culture technique and identified as the genus Rhodococcus by morphological, cultural, biochemical characteristics, and Biolog system. The optimal medium composition and cultural conditions for the growth and degradation of phenol by Rhodococcus sp. P2 were 0.1% of (NH4)2SO4, 0.2% of KH2PO4, 0.25% of Na2HPO4ㆍ12H2O, 0.2% of MgSO4ㆍ7H2O, and 0.008% of CaCl2ㆍ2H2O along with initial pH 8.5 at 30℃. Rhodococcus sp. P2 could grow with phenol as the sole carbon source up to 1,800 ppm in batch cultures, but did not grow in medium containing above 2,000 ppm of phenol. When 800 ppm phenol was given in the optimal media, Rhodococcus sp. P2 completely degraded it within 24 h. Meanwhile, 1,800 ppm of phenol was degraded within 9 days. Rhodococcus sp. P2 could utilize toluene, n-hexane, xylene and benzene as sole carbon source.

Key words: Phenol, Biodegradation, Biological treatment

각 key word의 첫 글자만 대문자(고유명사, 약어 제외)

*Corresponding Author: Gil-Dong Hong, Department of Environmental Engineering, Sekye University, Busan 47514, Korea

Phone : ＋82-51-510-1752

E-mail : kenss@hanmail.net

서 론

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Table 1. The effect of nitrogen source on growth and phenol degradation

of Rhodococcus sp. L5

Nitrogen source	34 hour		48 hour
	Growth (A660)	Residual phenol (ppm)	Growth (A660)	Residual phenol (ppm)
None	0.053	1000	0.071	1000
Ammonium acetate	0.998	555	1.585	0
NH4Cl	0.524	705	1.229	0
NH4NO3	0.766	766	1.179	0
(NH4)2SO4	0.651	475	1.182	0
KNO3	0.294	879	1.175	0

영문만 사용 가능, 좌우측 수직라인 존재 유무, 수평라인 스타일 등 확인

영문만 사용 가능

Fig. 1. Effect of feather concentration on feather degradation and keratinolytic enzyme production by B. megaterium F7-1. ●, keratinolytic activity; ■, feather degradation.

감사의 글

본 논문은 OOOOO의 지원에 의해 수행되었습니다.

REFERENCES

Hong, K. D., Kim, C. S., Lee, Y. H., 2010, Characteristics of air pollution in the presence of land and sea breeze numerical simulation, Atmos. Env., 17, 35-42.

참고문헌 인용방법의 자세한 내용은 [투고규정]에서 확인!

∙Professor. Gil-Dong Hong

Department of Environmental Engineering, Sekye University

kenss@hanmail.net

∙Doctor’s course. Mong-Yong Lee

Department of Biotechnology, Sekye University

kenss@hanmail.net

∙Graduate student. Chun-Hyang Seong

Department of Chemical Engineering, Sekye University

kenss@hanmail.net

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